Refine your search
Collections
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Rajender, G.
- Sensitive and Validated HPLC Method for Determination of Paclitaxel in Human Serum
Abstract Views :336 |
PDF Views:143
Authors
Affiliations
1 Research & Development Division, Relisys Medical Devices Ltd (RMDL), Hyderabad-501 510, Andhra Pradesh, IN
1 Research & Development Division, Relisys Medical Devices Ltd (RMDL), Hyderabad-501 510, Andhra Pradesh, IN
Source
Indian Journal of Science and Technology, Vol 2, No 5 (2009), Pagination: 52-54Abstract
Determination of paclitaxel in human blood sample by HPLC method using carbamazepine as internal standard is described. The absolute recovery of paclitaxel from human serum was >90% and the limit of quantification was 0.05 µg/ml. The intra-day relative standard deviation (RSD) ranged from 1. 02 to 3.73% at 1.0 µg/ml, 1.16 to 5.03% at 10.0 µg/ml and 3.33 to 5.01% at 50.0 µg/ml. The inter-day RSD were 1.03, 2.16 and 1.95% at 1.0, 10.0, 50.0 µg/ml, respectively. The method can be applied to measure the serum concentration of paclitaxel in pharmacokinetic and elution kinetics.Keywords
Paclitaxel, Human Blood Sample, Serum, HPLCFull Text
References
- Heldman A Cheng L, Jenkins G, Heller P, Kim D, Ware M, Nater C, Hruban R, Rezai B, Abella B, Bunge K, Kinsella J, Sollott S, Lakatta E, Brinker J, Hunter W, and Froehlich J (2001) Paclitaxel stent coating inhibits neointimal hyperplasia at 4 weeks in a porcine model of coronary restenosis. Circulation. 103, 2289–95.
- Kuhn J, Rizzo J, Chang S, Schold C,Spence A, Berger M, Robbins H, Metha M, Bozik M, Fulton D, Rector D and Prodos M (1997) Effect of anticinvulsants (ACs) on the phromacokinetics (PK) and metabolic profile of Paclitaxel. Am. Soc. Clin. Oncol. 16, 224a.
- Kumar N (1981) Taxol-induced Polymerization of Purified Tubulin. J. Biological. Chem. 256, 10435-10441.
- Nannan Panday VR, Meerum Terwot JM and Ten Bokkel Huinink WW (1998) The role of pro drug therapy in the treatment of cancer. Proc. Am. Soc. Clin. Oncol. 17,742a.
- Patton JF, Suzanne J, Fanthony G, Haimworth J, Willent N, Baker, Bechert J, Lacerna L and Howard Buris (2000) Weekly paclitaxel prolongated oral eniluracil/ 5-flurouracil: results of a phase-׀ combination study. Proc. Am. Soc. Clin. Oncol. 19,746a.
- Rinaldi, DA, Lormand N, Brierre J, Cole J, Stagg P, Fontenot F, Buller E and Rainer J (2000) A phase 1 trial of gemcitabine (GEM) and paclitaxel (PAc) in patients with advanced solid tumors, administered every 21 days (LOA-2). Proc. Am. Soc. Clin. Oncol. 19, 848a.
- Schellens JHM, Meeru JM, Bokke HWW, Rosing H, Tellingen O, Swart M, Duchin KL and Beijner JH (1998) Cyclosporin A (CsA) strongly enhances oral bioavailability of paclitaxel (Pac) in cancer patients. Pro. Am. Soc. Clin. Oncol. 17, 717a.
- Schwartz GK, Kaubish A, Saltz L and Lison D (2000) Phase 1 trial of sequential paclitaxel and the cyclin dependent kinase inhibitor flavopiridol. Proc. Am. Soc. Clin. Oncol. 18, 160a.
- Sharma A, Conway WD and Staudinger RM (1994) Reverse-phase highperformance liquid chromatographic determination of taxol in mouse plasma. J. Chromatogr. 655, 315-319.
- Sparreboom A and Van Tellingen O (1998) Preclinical pharmacokinetics of paclitaxel and docetaxel. Anti Cancer Drugs. 8, 1-17.
- Wani M, Taylor H, Wall M, Coggon P and McPhail A (1971) Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J.Am. Chem. Soc. 93, 2325–2327.
- A Docile Method of Isolation and Purification of Riboflavin Binding Protein (Rfbp) from Peahen (Pavo cristatus) and Domestic Fowl (Gallus gallus)
Abstract Views :907 |
PDF Views:117
Authors
Affiliations
1 Dept of Zoology, & Dept of Bio- chemistry, Kakatiya University, Warangal-506009, IN
1 Dept of Zoology, & Dept of Bio- chemistry, Kakatiya University, Warangal-506009, IN
Source
Indian Journal of Science and Technology, Vol 2, No 11 (2009), Pagination: 32-34Abstract
Riboflavin binding protein (Rfbp) was isolated from domestic fowl (Gallus gallus) and peahen (Pavo cristatus) egg-white and egg-yolk. The protein was purified in two steps, DEAE-Sephadex A-50 ion exchange chromatography and eluted with phosphate buffer pH 7.3 containing 0.5 M sodium chloride. The final purification of protein was achieved on Sephadex G-100. The purity of the protein was judged on cylindrical and slab gel electrophoresis, SDS-PAGE technique. Sephadex G-100 fraction Rfbp moved as a single band both on the Slab and Cylindrical gels. Comparison of the mobility of Rfbp with that of the standard molecular weight marker proteins revealed with that the Rfbp had a molecular weight close to 29,000 kd. Interestingly, hen egg-white Rfbp and peahen egg- white, yolk Rfbp had the same molecular weight as revealed by the SDS-PAGE. This is a novel approach for the study of riboflavin binding protein purified from different avian eggs in two steps and studied electrophoretic characterization with standard molecular weight marker.Keywords
Rfbp Purification Method, Peahen, Hen, Egg White-yolk, SDS, PAGE/nativeFull Text
References
- Abe T, Muto Y and Hosoya Hl (1975) Vitamin A transport in chicken plasma: isolation and characterization of retinolbinding protein (RBP), prealbumin (PA), and RBP-PA complex. J. Lipid Res. 16, 200-210.
- Booher LE (1933) The concentration and probable chemical nature of Vitamin G. J.Biol. Chem. 39, 102.
- Burch HB, Bessey OA and Lowry OH (1948) Flurometric measurements of Riboflavin and its natural derivatives in small quantities of blood serum and cells. J.Biol.Chem. 175, 457.
- Coates ME (1971) Physiology and Biochemistry of the Domesting Fowl (Eds. Bell D J & Freeman BM) Academic Press. New York. 1, pp: 373-392.
- Common RH, Bolton W and D Rutledge WA (1947-8) The influence of gonadal hormones on the composition of the blood and urine of the domestic fowl. J. Endocrinol. 5, 263.
- Dadd RH (1985) Comparative insect physiology, biochemistry and pharmacology (Eds. Krekut GA & Gillbert LI) Pergamum Press, New York. 4. pp: 313-390.
- Edelstein S, Lawson DEM and Kodicek E (1973) The transporting proteins of cholecalciferol and 25- hydroxycholecalciferol in serum of chicks and other species. Partial purification and characterization of the chick proteins. Biochem. J. 135, 417-426.
- Farrell HM, Mallette Jr, Buss FM and Clagett EG (1969) The nature of the Biochemical lesion avian renal riboflavinuria. 3. The isolation and characterization of the riboflavin binding protein from Egg albumin. Biochem. Biophys. Acta. 194, 433-442.
- Grasbeck R (1969) Instrinstic factor and the other vitamin B12 transport proteins. Prog. Hematol. 6, 233-260. 10. Hamazume Y, Mega T and Ikenaka T (1984) Yolk-riboflavin binding proteins and amino acid sequence of egg white riboflavin binding protein. J. Biochem. 95,1633-1644.
- Kanai M, Raz A and Goodman DWS (1968) Retinol-binding protein: the transtport protein for vitamin A in human plasma. J. Clin. Invest. 47, 2025-2044.
- Leammli UK (1979) Cleavage of structural proteins during the assembly of the head of bacteriophase T4. Nature. 227, 680-685.
- Naber EC, Cravens WW, Baumann CA and Bird HR (1954) The effect of thiamine analogs on embryonic development and growth of the chick. J. Nutr. 54,579-591.
- Ostrowski W, Skarzynski B and Zak Z (1962) Isolation and properties of flavoprotein from the egg yolk. Biochem. Biophysics. Acta. 59, 515-519.
- Rajender G, Benarjee G and Prasad MSK (2007) Purification and characterization of riboflavin binding protein in egg-white of Peacock (Pavo cristatus). Cur. Sci. 93, 24-25.
- Rhodes MB, Bennett N and Feeney RE (1959) The flavoprotein apoprotein system of egg white. J. Biol. Chem. 234, 2054-2060.
- Sonneborn DW and Hansen HJ (1970) Vitamin B12 binders of chicken serum and chicken proventruculus are immunologically similar. Science. 168, 591-592. 18. Thomas WC, Morgan HG, Conner TB, Haddock L, Bills CE and Howard JkE (1959) Studies of antiricketic activity in sera from patients with disorders of calcium metabolism and preliminary observations on the mode of transport of vitamin d in human serum. J. Clin. Invest. 38, 1078-1085.
- Winter WP, Buss EG, Clagett CO and Boucher RV (1967) the nature of the biochemical lesion in avian renal riboflavinuria. II. The inherited change of a riboflavinbinding protein from blood and eggs. Comp. Biochem. Physiol. 22, 897-906.
- Yagi T and Maruyama K (1971) Purification and properties of cytochrome c3 of Desulfovibrio vulgaris, Miyazki. Biochim. Biphys. Acta. 243, 214.
- Young DW (1986) the biosynthesis of the vitamins thiamin, riboflavin, and folic acid. Nat. Prod. Rep. 3, 395-419.